New distributional records (74) of larval fishflies are reported for Kentucky. Twenty-five new county records were added for Nigronia serricornis (Say), and forty-two new county records were added for N. fasciatus (Walker), the most widely distributed fishfly in Kentucky (54 counties). These two species were sympatric in 14 streams in eastern Kentucky. One new county record was added for Neohermes concolor (Davis). Four new county records were noted for Chauliodes pectinicornis (Linnaeus), while two new county records were added for C. rastricornis Rambur. Following this investigation, the statewide distribution of fishfly species is as follows: N. serricornis (51 counties), N. fasciatus (54 counties), N. concolor (11 counties), C. pectinicornis (9 counties), and C. rastricornis (9 counties). Emergence patterns are mentioned for the five species of fish-flies in Kentucky.